Transcriptional regulation of Nostoc uptake hydrogenase
نویسندگان
چکیده
منابع مشابه
Transcriptional regulation of the uptake [NiFe]hydrogenase genes in Rhodobacter capsulatus.
Transcription of the hupSL genes, which encode the uptake [NiFe]hydrogenase of Rhodobacter capsulatus, is specifically activated by H(2). Three proteins are involved, namely the H(2)-sensor HupUV, the histidine kinase HupT and the transcriptional activator HupR. hupT and hupUV mutants have the same phenotype, i.e. an increased level of hupSL expression (assayed by phupS::lacZ fusion) in the abs...
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The overall processes of transcription and its regulation have advanced significantly in the last years, making our understanding of prokaryotic biology more complex and detailed. In fact, a systematic study of different aspects of transcriptional regulation opens up outstanding opportunities to improve and develop the perception of complex reaction mechanisms, genetic processes and cell functi...
متن کاملTranscriptional and mutational analysis of the uptake hydrogenase of the filamentous cyanobacterium Anabaena variabilis ATCC 29413.
A 10-kb DNA region of the cyanobacterium Anabaena variabilis ATCC 29413 containing the structural genes of the uptake hydrogenase (hupSL) was cloned and sequenced. In contrast to the hupL gene of Anabaena sp. strain PCC 7120, which is interrupted by a 10.5-kb DNA fragment in vegetative cells, there is no programmed rearrangement within the hupL gene during the heterocyst differentiation of A. v...
متن کاملTranscriptional regulation of Nostoc hydrogenases: effects of oxygen, hydrogen, and nickel.
The transcription of structural genes encoding two hydrogenases in N(2)-fixing cultures of the cyanobacteria Nostoc muscorum and Nostoc sp. strain PCC 73102 were examined by reverse transcription-PCR. A low level of oxygen and addition of nickel induce higher transcript levels of both hydrogenases, whereas molecular hydrogen has a positive effect on the transcription of the genes encoding only ...
متن کاملMolecular characterization of uptake hydrogenase in Frankia.
A molecular characterization of uptake hydrogenase in Frankia was performed by using two-dimensional gel electrophoresis, matrix-assisted laser-desorption ionization-time-of-flight mass spectrometry, PCR amplification and Southern blotting. A polypeptide of approx. 60 kDa was recognized in Frankia UGL011102, AVCI1 and KB5 on the two-dimensional gel by blotting with Ralstonia eutropha (Hox G) an...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1999
ISSN: 0378-1097
DOI: 10.1016/s0378-1097(98)00527-8